Analysis of Thr aminoacyl synthetase.

Analysis performed on 2024-07-10 11:40:15

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 45268 593 44675 26467
Archaea 3397 114 3283 1424
Vertebrata 2692 1009 1683 465
Mammals 1071 128 943 134

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
723 8 607

Domains

Domains
Current Study 1, 76
Guo et al (2010) 1, 80

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 648 75 723 8.640000
Number of Variants 555 60 615 9.250000
Number of Pathogenic Variants 7 1 8 7.000000
Number of Benign/Unknown Variants 548 59 607 9.288136
Fisher exact test 0.562290

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 643 80 723 8.037500
Number of Variants 553 62 615 8.919355
Number of Pathogenic Variants 7 1 8 7.000000
Number of Benign/Unknown Variants 546 61 607 8.950820
Fisher exact test 0.574829

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 648 75 0.90 (0.00) 0.68 (0.02) 9.481e-01 6.905e-01 41827.00 6.73e-25
Guo et al (2010) 643 80 0.90 (0.00) 0.68 (0.02) 9.488e-01 6.912e-01 44275.00 2.99e-26

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains